UHPLC systems have been engineered with sub-2 particle, high-pressure applications in mind, resulting in complex pump redesigns that add significantly to system cost. The chromatograms shown in the figure demonstrate the potential of Kinetex HPLC columns to reduce analysis time, compared to traditional 5 μm fully porous particles, without compromising analyte resolution or generating extreme backpressures. Coreshell particles can be used with high mobile phase flow rates to further reduce analysis time without significant losses in separation efficiency, whereas the performance of fully porous particles begins to drop off sharply at high flow rates. Solution: Kinetex™ core-shell HPLC particle technologies provide a high-efficiency, low-pressure solution for laboratories looking to increase productivity and decrease costs without investing in a UHPLC system.
Unfortunately, columns packed with sub-2 μm particles typically generate pressures that exceed the limits of standard HPLC instruments and require the use of ultra-high pressure HPLC systems, which can be cost-prohibitive. Significant improvements have been made in the preparation of sub-2 μm HPLC packing materials, which provide high-efficiency separations in less time when packed in shorter columns. This can be accomplished in part by leveraging higher efficiency HPLC columns to increase analysis speed. Problem: Chromatographers have an ongoing need to increase productivity and decrease costs.
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